EFFECT OF DIETARY FISH OIL ON DEVELOPMENT AND SELECTED FUNCTIONS OF MURINE INFLAMMATORY MACROPHAGES

Inflammatory macrophages from mice fed diets containing menhaden fish oil (MFO) have a reduced capacity for cytotoxicity of mastocytoma cells upon activation with interferon-gamma (IFN-gamma) and lipopolysaccharide due to an altered responsiveness to IFN-gamma. In an effort to elucidate further how dietary MFO effects macrophage function, we have studied the maturation of inflammatory macrophages from mice fed MFO compared with mice fed safflower oil (SFO) using several processes that serve as markers of the activational state. No significant differences in the recruitment or percentage of peritoneal exudate cells as macrophages after thioglycollate injection and no differences in spreading, binding, or phagocytosis of sheep erythrocytes or phagocytosis of yeast by inflammatory macrophages were observed when the dietary groups were compared. However, MFO macrophages had an altered capacity for peroxide release when stimulated with unopsonized zymosan (10-200-mu-g/ml). Furthermore, to elucidate how MFO feeding could alter IFN-gamma-induced responses of inflammatory macrophages, we assessed phorbol-12-myristate-13-acetate-induced hydrogen peroxide production and expression of class II MHC determinants (Ia). There were no differences between macrophages from mice fed the two diets with respect to the production of peroxide when they were preincubated with 0.1-10 U/ml of IFN-gamma. However, MFO macrophages had greater peroxide production after enhancement with 100 U/ml of IFN-gamma. With respect to Ia induction, the percentage of macrophages responding to IFN-gamma was not altered by diet, and there were no differences in expression of Ia induced by 24 hr exposure to IFN-gamma. Thus the differential effect of MFO compared with SFO is probably mediated not by an alteration in the maturation of inflammatory macrophages but rather through the alteration of IFN-gamma-induced functions such as peroxide production.