SEQUENTIAL MEASUREMENTS OF GLYCOLYTIC AND TRICARBOXYLIC-ACID CYCLE METABOLITES IN SINGLE SAMPLE ALIQUOTS

被引:7
|
作者
COLSON, C
KLAPPER, MH
机构
关键词
D O I
10.1016/0003-2697(79)90091-5
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Standard, pyridine nucleotide-linked fluorometric assays were sequenced so that 14 glycolytic and tricarboxylic acid cycle intermediates [from the water mold, Blastocladiella emersonii] can be measured in only 2 sample aliquots. This multiple assay procedure, which is based on commercially available enzymes and reagents, allows analysis on small tissue samples in relatively short times.
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页码:394 / 399
页数:6
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