GENOTYPIC COMPARISON OF HEAT-SHOCK PROTEIN-SYNTHESIS IN SORGHUM

被引：7
|
作者：
JORGENSEN, JA
ROSENOW, DT
NGUYEN, HT
机构：
[1] TEXAS TECH UNIV,MAIL STOP 2122,LUBBOCK,TX 79409
[2] TEXAS AGR EXPTL STN,LUBBOCK,TX 79401
来源：
CROP SCIENCE | 1993年 / 33卷 / 03期
关键词：
D O I：
10.2135/cropsci1993.0011183X003300030042x
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
High temperature stress is an environmental factor limiting sorghum (Sorghum bicolor (L.) Moench) productivity. We determined the optimum heat-shock protein (HSP) induction temperature in sorghum leaves and investigated intraspecific genetic variability in HSP synthesis among inbred lines RTx430, SC599-6, and SC326-6. One dimensional electrophoresis indicated an optimum 42-degrees-C synthesis temperature in seedling leaves. Analysis of genetic variation by two-dimensional electrophoresis showed only three genotype-specific but many common HSPs. Sixteen of the common HSPs were synthesized in control samples; however, synthesis during high temperatures was dramatically enhanced. The results suggest limited variation in HSP synthesis and predominant constitutive HSP synthesis among these sorghum lines.
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页码：638 / 641
页数：4
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