RESEARCH ON THE ISOLATION OF GENOMIC DNA FROM OLD APPLE VARIETIES

The apple scab disease has probably evolved over a long time along with the apples. The disease is caused by the fungus Venturia inaequalis (Cke.) Wint, anamorph Spilocaea pomi Fr. The aim of this study were the isolation and quantification the genomic DNA on old Romanian varieties in order to select the most important to them for the Marker Assisted Selections (MAS) on apple trees. This paper presents the results of DNA isolation by exploring local populations of apple like 'Prescurate', 'Seghese', 'Viesti' and 'Knis' apples old varieties, that are well adapted to the conditions in Romania and that are un interesting genetic potential for resistance to scab. Only the old apple varieties were used in the study. The implementation of marker selection depends on the quality and quantity of isolated genomic DNA. The results of the quantification performed following genomic DNA isolation show a large variability in the amount of DNA in each old apple variety. The DNA concentration in apple varieties both parents and some descendants have shown that the values are higher in parents than hybrids, with the highest value for 'Turnu' variety with 179.1 ng / mu l, followed by 'Calvil alb' variety with 161.1 ng / mu l, then 'Varga' variety with 155.0 ng / mu l.