BACULOVIRUS-MEDIATED PRODUCTION OF THE HUMAN GROWTH-HORMONE IN LARVAE OF THE SILKWORM, BOMBYX-MORI

被引：28

作者：

KADONOOKUDA, K ^{[1
]}

YAMAMOTO, M ^{[1
]}

HIGASHINO, Y ^{[1
]}

TANIAI, K ^{[1
]}

KATO, Y ^{[1
]}

CHOWDHURY, S ^{[1
]}

XU, JH ^{[1
]}

CHOI, SK ^{[1
]}

SUGIYAMA, M ^{[1
]}

NAKASHIMA, K ^{[1
]}

MAEDA, S ^{[1
]}

YAMAKAWA, M ^{[1
]}

机构：

[1] NATL INST SERICULTURAL & ENTOMOL SCI,BIOL DEF LAB,TSUKUBA,IBARAKI 305,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 213卷 / 02期

关键词：

D O I：

10.1006/bbrc.1995.2144

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To express the cDNA encoding human growth hormone (hGH) in larvae of Bombyx mori, B. mori nuclear polyhedrosis virus (BmNPV) was employed as an expression vector. For the construction of the recombinant virus, the hGH cDNA was inserted into the downstream of the strong polydedrin promoter to achieve a high level expression. Immunoblot analysis revealed that the virus-mediated hGH was synthesized in the larvae and secreted into the hemolymph. The yield of the recombinant hGH synthesized in the larvae reached to a level of 160 mu g/ml of hemolymph after purification. The purified recombinant hGH was confirmed to have both the same molecular weight and amino acid sequence at its N-terminal region as those of the natural counterpart. In addition, the biological activity of the recombinant hGH was comparable to that of the natural hGH in the growth-stimulating effect on rat Nb 2 Node lymphoma cells. (C) 1995 Academic Press, Inc.

引用

页码：389 / 396

页数：8

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