Molecular typing of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from Shiraz teaching hospitals by PCR-RFLP of coagulase gene

被引：1
|
作者：
Khoshkharam-Roodmajani, Hossein [1 ,2 ]
Sarvari, Jamal [1 ]
Bazargani, Abdollah [1 ]
Kandekar-Ghahraman, Mohammad Reza [1 ]
Nazari-Alam, Ali [1 ]
Motamedifar, Mohammad [1 ,3 ]
机构：
[1] Shiraz Univ Med Sci, Fac Med, Dept Bacteriol & Virol, Shiraz, Iran
[2] Shiraz Univ Med Sci, Student Res Comm, Shiraz, Iran
[3] Shiraz Univ Med Sci, Shiraz HIV AIDS Res Ctr, Shiraz, Iran
来源：
IRANIAN JOURNAL OF MICROBIOLOGY | 2014年 / 6卷 / 04期
关键词：
Antibiotic resistance; MRSA; PCR; RFLP; Coagulase gene;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Background and Objectives: To investigate coagulase gene polymorphisms of MRSA and MSSA isolates from Shiraz teaching hospitals from 2011 to 2012. Materials and Methods: A total of 302 isolates of Staphylococcus aureus were collected from clinical specimens in three major teaching hospitals and confirmed on the basis of morphological characteristics and biochemical tests. The isolates were subjected to molecular typing on the basis of coagulase enzyme gene polymorphism by PCR-RFLP. Results: There were 27 and 28 different RFLP patterns for AluI and HaeIII restriction enzymes respectively. This study showed that the discriminatory power of coagulase gene typing by Hae III enzyme was more than that of Alu I enzyme. Conclusion: PCR-RFLP method is rapid, reproducible, simple and efficient for typing Staphylococcus aureus isolated from clinical specimens. This study showed that Hae III discriminatory power is better than Alu I for typing Staphylococcus aureus isolates.
引用
收藏
页码：246 / 252
页数：7
相关论文
共 50 条
  • [1] Analysis of methicillin-resistant and methicillin-susceptible Staphylococcus aureus by a molecular typing method based on coagulase gene polymorphisms
    Kobayashi, N
    Taniguchi, K
    Kojima, K
    Urasawa, S
    Uehara, N
    Omizu, Y
    Kishi, Y
    Yagihashi, A
    Kurokawa, I
    [J]. EPIDEMIOLOGY AND INFECTION, 1995, 115 (03): : 419 - 426
  • [2] MOLECULAR TYPING OF METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS STRAINS BY PCR-RFLP OF SPA GENE
    Aslanimehr, Masume
    Peymani, Amir
    Yaghoobfar, Rezvan
    Tavakoli, Mahnaz
    [J]. IRANIAN JOURNAL OF PUBLIC HEALTH, 2014, 43 : 265 - 265
  • [3] CHARACTERIZATION OF METHICILLIN-SUSCEPTIBLE AND METHICILLIN-RESISTANT STAPHYLOCOCCUS-AUREUS ISOLATES BY MOLECULAR TYPING METHODS
    KAUFHOLD, A
    LIVDAHL, C
    FERRIERI, P
    [J]. ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES, 1992, 277 (03): : 309 - 319
  • [4] Rapid molecular typing of methicillin resistant Staphylococcus aureus by PCR-RFLP
    Wichelhaus, TA
    Hunfeld, KP
    Böddinghaus, B
    Kraiczy, P
    Schäfer, V
    Brade, V
    [J]. INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2001, 22 (05): : 294 - 298
  • [5] Molecular characterization and susceptibility of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from hospitals and the community in Vladivostok, Russia
    Baranovich, T.
    Zaraket, H.
    Shabana, I. I.
    Nevzorova, V.
    Turcutyuicov, V.
    Suzuki, H.
    [J]. CLINICAL MICROBIOLOGY AND INFECTION, 2010, 16 (06) : 575 - 582
  • [6] Distribution of Aminoglycoside-Modifying Enzymes and Molecular Analysis of the Coagulase Gene in Clinical Isolates of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus
    Seyedi-Marghaki, Farhad
    Kalantar-Neyestanaki, Davood
    Saffari, Fereshteh
    Hosseini-Nave, Hossein
    Moradi, Mohammad
    [J]. MICROBIAL DRUG RESISTANCE, 2019, 25 (01) : 47 - 53
  • [7] Usefulness of PCR-RFLP coa gene for clonal classification of methicillin-resistant Staphylococcus aureus isolates in tertiary hospitals
    Gonzalez-Dominguez, Maria
    Potel, Carmen
    Seral, Cristina
    Constenla, Lucia
    Castillo, F. Javier
    Alvarez, Maximiliano
    [J]. SCANDINAVIAN JOURNAL OF INFECTIOUS DISEASES, 2014, 46 (10) : 719 - 722
  • [8] Reversion of Methicillin-Resistant Staphylococcus aureus Skin Infections to Methicillin-Susceptible Isolates
    Patel, Anisha B.
    Hill, Emma
    Simpson, Eric L.
    Hanifin, Jon M.
    [J]. JAMA DERMATOLOGY, 2013, 149 (10) : 1167 - 1171
  • [9] Molecular epidemiology of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from global clinical trials
    Goering, Richard V.
    Shawar, Ribhi M.
    Scangarella, Nicole E.
    O'Hara, F. Patrick
    Amrine-Madsen, Heather
    West, Joshua M.
    Dalessandro, Marybeth
    Becker, Julie A.
    Walsh, Stacey L.
    Miller, Linda A.
    van Horn, Stephanie F.
    Thomas, Elizabeth S.
    Twynholm, Monique E.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (09) : 2842 - 2847
  • [10] Molecular typing of methicillin-resistant Staphylococcus aureus strains by PCR-RFLP of SPA gene: A reference laboratory perspective
    Mehndiratta, P. L.
    Bhalla, P.
    Ahmed, A.
    Sharma, Y. D.
    [J]. INDIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 27 (02) : 116 - 122
12345