A NOVEL MEDIATOR BETWEEN ACTIVATOR PROTEINS AND THE RNA POLYMERASE-II TRANSCRIPTION APPARATUS

被引：363
|
作者：
KELLEHER, RJ
FLANAGAN, PM
KORNBERG, RD
机构：
[1] Department of Cell Biology Fairchild Center Stanford University School of Medicine Stanford
来源：
CELL | 1990年 / 61卷 / 07期
关键词：
D O I：
10.1016/0092-8674(90)90685-8
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
One gene activator protein may interfere with the effects of another in eukaryotic cells. We report here that a hybrid yeast-herpes gene activator protein inhibits transcriptional activation by a thymidine-rich DNA element in yeast. This example of activator interference can be faithfully reproduced in vitro. Interference is reversed by a partially purified yeast component, but not by RNA polymerase II or various polymerase II transcription factors. We conclude that the partially purified yeast component is a novel factor, and we suggest this factor mediates the transcriptional activation process. © 1990.
引用
收藏
页码：1209 / 1215
页数：7
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