EFFECT OF GOSSYPOL ON TRYPOMASTIGOTES AND AMASTIGOTES OF TRYPANOSOMA-CRUZI

被引：21

作者：

ROVAI, LE ^{[1
]}

AOKI, A ^{[1
]}

DEBURGOS, NMG ^{[1
]}

BLANCO, A ^{[1
]}

机构：

[1] NATL UNIV CORDOBA,FAC CIENCIAS MED,CTR MICROSCOPIE ELECTR,RA-5016 CORDOBA,ARGENTINA

来源：

JOURNAL OF PROTOZOOLOGY | 1990年 / 37卷 / 04期

关键词：

Chagas disease; gossypol inactivation by proteins; trypanocidal agent; trypomastigote motility; ultrastructural changes; Vero cell cultures;

D O I：

10.1111/j.1550-7408.1990.tb01148.x

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Bloodstream Trypanosoma cruzi trypomastigotes isolated from infected mice undergo reduction of motility and structural damages after 5 to 45 min exposure to gossypol at concentrations ranging from 5 to 50 μM. When 1% serum albumin is added to the incubation medium, no alterations of parasites are observed, even with 100 μM gossypol. Intracellular T. cruzi amastigotes in infected Vero cell cultures exposed to 5 μM gossypol for 2 h do not show changes. Incubation with 5 μM gossypol for 48 h produces complete disruption of host cells; however, the amastigotes they contain show only mineor alterations. The observations indicate that, in protein‐rich media, gossypol is complexed into associations which have no activity on the different forms of the T. cruzi biological cycle. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：280 / 286

页数：7

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