METABOLISM OF ESTROGENS INVIVO AND INVITRO BY PHASEOLUS-VULGARIS

被引：11

作者：

YOUNG, IJ ^{[1
]}

KNIGHTS, BA ^{[1
]}

HILLMAN, JR ^{[1
]}

机构：

[1] UNIV GLASGOW, DEPT BOT, GLASGOW G12 8QQ, SCOTLAND

来源：

ZEITSCHRIFT FUR PFLANZENPHYSIOLOGIE | 1979年 / 94卷 / 04期

关键词：

D O I：

10.1016/S0044-328X(79)80114-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Seedlings of P. vulgaris cv. Canadian Wonder were incubated with 4-14C-estrone, 4-14C-estradiol-17.beta. and 6,7-3H-estrone sulphate. Evidence from TLC, column chromatography on Lipidex, radio-GLC and crystallization to constant specific activity suggests that estrone and estradiol-17.beta. are interconvertible in plant tissues. A tissue homogenate supplemented with cofactors evidently can metabolize estrone. Analysis by gas chromatography and combined gas chromatography-mass spectrometry demonstrated unequivocally that the major product was estradiol. These data represent the 1st evidence from full mass spectrometry that estrogens can be metabolized by plant tissues.

引用

页码：307 / 316

页数：10

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