PURIFICATION OF ADENOSINE-DEAMINASE FROM CHICKEN-EGG YOLK BY AFFINITY COLUMN CHROMATOGRAPHY

被引：4
|
作者：
LOPEZ, R
CABRE, F
FRANCO, R
CASCANTE, M
CANELA, EI
机构：
[1] Department of Biochemistry and Physiology, University of Barcelona Marti i Franques, 1. 08071, Barcelona, Catalonia
来源：
PREPARATIVE BIOCHEMISTRY | 1990年 / 20卷 / 3-4期
关键词：
D O I：
10.1080/00327489008050196
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Adenosine deaminase (adenosine aminohydrolase; E.C. 3.5.4.4) has been purified 4686-fold from egg yolk. The procedure developed was used to isolate the enzyme from eight chicken eggs. An easily prepared affinity column employing purine riboside was used as the final step in the purification. The method developed permits the rapid isolation and a high recovery of the protein. The specific activity of the enzyme preparation obtained is 81.4 mU/mg.
引用
收藏
页码：199 / 204
页数：6
相关论文
共 50 条
  • [1] PURIFICATION OF HUMAN ERYTHROCYTE ADENOSINE-DEAMINASE BY AFFINITY COLUMN CHROMATOGRAPHY
    SCHRADER, WP
    STACY, AR
    POLLARA, B
    JOURNAL OF BIOLOGICAL CHEMISTRY, 1976, 251 (13) : 4026 - 4032
  • [2] AN AFFINITY COLUMN FOR ADENOSINE-DEAMINASE
    DANTON, MJ
    COLEMAN, MS
    FEDERATION PROCEEDINGS, 1983, 42 (07) : 2047 - 2047
  • [3] PREPARATIVE PURIFICATION OF ADENOSINE-DEAMINASE FROM HUMAN ERYTHROCYTES BY AFFINITY-CHROMATOGRAPHY
    ARAN, JM
    CANELA, EI
    FRANCO, R
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1990, 532 (01): : 75 - 85
  • [4] HUMAN PLACENTAL ADENOSINE-DEAMINASE CONVERSION PROTEINS - PURIFICATION AND USE IN AFFINITY CHROMATOGRAPHY
    TROTTA, PP
    YAMAURA, I
    BALIS, ME
    FEDERATION PROCEEDINGS, 1978, 37 (06) : 1341 - 1341
  • [5] THE CHARACTERIZATION OF AN ADENOSINE-DEAMINASE FROM CHICKEN SERUM
    ROKOSU, AA
    COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY, 1983, 74 (03): : 441 - 444
  • [6] AFFINITY LABELING OF ADENOSINE-DEAMINASE
    SEGNINI, D
    LUCACCHINI, A
    ITALIAN JOURNAL OF BIOCHEMISTRY, 1977, 26 (05): : 356 - 357
  • [7] ISOLATION OF MUTANT ADENOSINE-DEAMINASE BY COFORMYCIN AFFINITY-CHROMATOGRAPHY
    DANTON, MJ
    COLEMAN, MS
    ANALYTICAL BIOCHEMISTRY, 1986, 159 (01) : 233 - 239
  • [8] GENERAL METHOD OF PURIFICATION OF ADENOSINE DEAMINASE BY AFFINITY CHROMATOGRAPHY
    ROSSI, CA
    LUCACCHINI, A
    MONTALI, U
    RONCA, G
    INTERNATIONAL JOURNAL OF PEPTIDE AND PROTEIN RESEARCH, 1975, 7 (01): : 81 - 89
  • [9] PURIFICATION AND CHARACTERIZATION OF MURINE ADENOSINE-DEAMINASE
    INGOLIA, DE
    YEUNG, CY
    RUDOLPH, FB
    KELLEMS, RE
    JOURNAL OF CELL BIOLOGY, 1983, 97 (05): : A421 - A421
  • [10] Affinity purification of immunoglobulins from chicken egg yolk using a new synthetic ligand
    Verdoliva, A
    Basile, G
    Fassina, G
    JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 749 (02): : 233 - 242
12345