INVIVO EFFECT OF ORTHOVANADATE ON THE NITRATE REDUCTASE-ACTIVITY IN CUCUMBER ROOTS

被引：0
|
作者：
MARCINIAK, J
BUCZEK, J
机构：
来源：
ACTA PHYSIOLOGIAE PLANTARUM | 1992年 / 14卷 / 01期
关键词：
CUCUMIS-SATIVUS L; INHIBITION OF NADH NITRATE REDUCTASE; NADHFR REDUCTASE; MVH NITRATE REDUCTASE; ORTHOVANADATE;
D O I：
暂无
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Sodium orthovanadate (Na3VO4) at 500-mu-M concentration, strongly inhibited a NADH-dependent nitrate reduction (NADH:NR activity, E.C. 1.6.6.1) and Mo-pterin component activity (MVH:NR activity) in the nitrate reductase complex extracted from cucumber roots. However, orthovanadate had no effect on dehydrogenase activity measured by the reduction of ferricyanide (NADH:FR activity). It was suggested that the Mo-pterin component in the nitrate reductase molecule could be a site of orthovanadate inactivation in enzyme complex.
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页码：49 / 54
页数：6
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