FLUORESCENT AND PHOTOAFFINITY-LABELING DERIVATIVES OF RHIZOXIN

被引：12

作者：

SAWADA, T ^{[1
]}

HASHIMOTO, Y ^{[1
]}

LI, Y ^{[1
]}

KOBAYASHI, H ^{[1
]}

IWASAKI, S ^{[1
]}

机构：

[1] UNIV TOKYO,INST APPL MICROBIOL,1-1-1 YAYOI,BUNKYO KU,TOKYO 113,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 178卷 / 02期

关键词：

MACROCYCLIC LACTONE ANTIBIOTICS; TUBULIN;

D O I：

10.1016/0006-291X(91)90144-V

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A fluorescent probe (D-RZX) and a photoreactive fluorescent probe (AD-RZX) for studying the rhizoxin binding site on tubulin were prepared by the derivatization of rhizoxin (RZX). D-RZX consists of a rhizoxin moiety and a dansyl moiety. AD-RZX has a 5-azidonaphthalene-1-sulfonyl moiety instead of the dansyl moiety of D-RZX. Both D-RZX and AD-RZX bound tubulin in a mutually competitive manner with rhizoxin, indicating their binding to the rhizoxin site on tubulin. AD-RZX bound the rhizoxin site covalently after UV-irradiation, thus showing its usefulness as a photoaffinity probe for labeling of the rhizoxin site. © 1991.

引用

页码：558 / 562

页数：5

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