Rabbit-antihuman IgG, yolk immunoglobulins (IgY) and monoclonal antibodies against Rickettsia conorii were adsorbed to colloidal gold. Thus obtained conjugates were found to be active and responded specifically to the agent of the Mediterranean Spotted fever. The suitability of the immunogold assay as a alternative to other known immunoenzymatic or immunofluorescent methods for assessing the presence and/or activity of rickettsial antigens is discussed.
机构:
WALTER REED ARMY INST RES, DEPT HAZARDOUS MICROORG, WASHINGTON, DC 20012 USAWALTER REED ARMY INST RES, DEPT HAZARDOUS MICROORG, WASHINGTON, DC 20012 USA
EISEMANN, CS
OSTERMAN, JV
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机构:
WALTER REED ARMY INST RES, DEPT HAZARDOUS MICROORG, WASHINGTON, DC 20012 USAWALTER REED ARMY INST RES, DEPT HAZARDOUS MICROORG, WASHINGTON, DC 20012 USA
机构:Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Center for Infectious Diseases, Public Health Service, United States Department of Health and Human Services, Centers for Disease Control, Atlanta, Georgia
机构:Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, Center for Infectious Diseases, Centers for Disease Control, U.S. Public Health Service, Department of Health and Human Services, Atlanta, Georgia