DETERMINATION OF NICOTINAMIDE AND PYRIDOXINE IN AN ELEMENTAL DIET BY COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH UV DETECTION

被引：16
|
作者：
IWASE, H
机构：
[1] Ajinomoto Co., Inc., Kawasaki-ku, Kawasaki, 210, Kawasaki Factory
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 625卷 / 02期
关键词：
D O I：
10.1016/0021-9673(92)85225-I
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The determination of nicotinamide and pyridoxine in an elemental diet containing 46 compounds was performed by column-switching high-performance liquid chromatography with UV detection at 260 and 290 nm, respectively. The method is simple, rapid, sensitive and reproducible. The calibration graphs for the two vitamins were linear in the ranges 0-0.2 and 0-0.015 mug, respectively. The recoveries of both vitamins by the standard addition method were over 95%. There was good agreement between the concentrations indicated and found for both vitamins.
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页码：377 / 381
页数：5
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