CHARACTERIZATION OF CRYSTALS OF A FAB FRAGMENT OF A MURINE MONOCLONAL-ANTIBODY

被引：2
|
作者：
BAN, N [1 ]
ESCOBAR, C [1 ]
DAY, J [1 ]
GREENWOOD, A [1 ]
LARSON, S [1 ]
MCPHERSON, A [1 ]
机构：
[1] IMMUNOPHARMACEUT INC,SAN DIEGO,CA 92127
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 222卷 / 03期
关键词：
CRYSTALS; FAB; X-RAY; ANTIBODY;
D O I：
10.1016/0022-2836(91)90484-N
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The Fab fragment of an antibody, made against an E2-specific feline infectious peritonitis virus neutralizing antibody, has been crystallized in a form suitable for X-ray diffraction analysis from PEG 4000 using vapor diffusion methods. The Fab fragment crystals diffract to about 2·9 Å resolution and are of triclinic space group P1. Unit cell dimensions, by which the reciprocal lattice can be indexed, are a = 57·16 A ̊, b = 70·85 A ̊, c = 75·81 A ̊, α = 85·11{ring operator}, β = 121·28{ring operator} and γ = 116·33{ring operator}. There are two Fab fragments comprising the asymmetric unit of the crystals. The presence of a pseudo-mirror plane in the diffraction pattern suggests the presence of at least an approximate dyad axis relating the two Fab fragments within the asymmetric unit. © 1991.
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收藏
页码：445 / 446
页数：2
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