PURIFICATION AND PARTIAL CHARACTERIZATION OF TRYPSIN-INHIBITORS FROM SEEDS OF CLITORIA-TERNATEA

被引：32
|
作者：
MACEDO, MLR
XAVIER, J
机构：
[1] Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Ceará, Fortaleza, Ceará, 60001, Caixa Postal 1065, Fortaleza
来源：
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1992年 / 58卷 / 01期
关键词：
CLITORIA-TERNATEA SEEDS; TRYPSIN INHIBITORS; KUNITZ; BOWMAN-BIRK;
D O I：
10.1002/jsfa.2740580110
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Three trypsin inhibitors of 20, 12 and 7 kDa were isolated in pure form from seeds of the vine Clitoria ternatea L of the tribe Glycinea of the Papilionoideae. The 20-kDa inhibitor is a one-chain molecule with arginine in the reactive site. The 12-kDa one is also a one-chain molecule and has lysine in the reactive site. It is also an inhibitor of chymotrypsin. They were tentatively assigned to the Kunitz and Bowman-Birk families, respectively. The small molecular weight inhibitor (7 kDa) also has an arginine in the reactive site and is probably of the Bowman-Birk type.
引用
收藏
页码：55 / 58
页数：4
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