EXPRESSION OF A HUMAN PROENKEPHALIN-A CDNA IN ESCHERICHIA-COLI

被引：0
|
作者：
MONSTEIN, HJ
机构：
[1] Dept. of Clinical Chemistry, Laboratory of Molecular Biology, Copenhagen, DK-2100
来源：
BIOSCIENCE REPORTS | 1990年 / 10卷 / 05期
关键词：
PROENKEPHALIN-A; RECOMBINANT PROTEIN; ESCHERICHIA-COLI EXPRESSION; VECTOR DNA;
D O I：
10.1007/BF01152293
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A 1000 base pair cDNA coding for the entire human proenkephalin A(proA) polypeptide was subcloned into the multifunctional pMPV 2911/M E. coli vector. The recombinant plasmid was found to express an approximately 30 kDa prohormone, which was recognized by a Met-Arg6-Phe2 antibody, directed against the C-terminal part of the enkephalin A prohormone. The expression of human proenkephalin A cDNA should thus permit the rapid purification of unfused recombinant enkephalin A prohormone, which itself may provide a model substrate to identify endoproteolytic processing activities.
引用
收藏
页码：461 / 467
页数：7
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