In vitro Analysis of Ubiquitin-like Protein Modification in Archaea

被引：1
|
作者：
Fu, Xian [1 ]
Adams, Zachary [1 ]
Maupin-Furlowl, Julie A. [1 ,2 ]
机构：
[1] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
[2] Univ Florida, Genet Inst, Gainesville, FL 32611 USA
来源：
BIO-PROTOCOL | 2018年 / 8卷 / 10期
基金：
美国食品与农业研究所;
关键词：
Archaea; Ubiquitin (Ub); Post-translational modification; SAMP;
D O I：
10.21769/BioProtoc.2845
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The ubiquitin-like (Ubl) protein is widely distributed in Archaea and involved in many cellular pathways. A well-established method to reconstitute archaeal Ubl protein conjugation in vitro is important to better understand the process of archaeal Ubl protein modification. This protocol describes the in vitro reconstitution of Ubl protein modification and following analysis of this modification in Haloferax volcanii, a halophilic archaeon serving as the model organism.
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页数：16
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