IMPROVEMENT OF AXIAL RESOLUTION IN CONFOCAL MICROSCOPY USING AN ANNULAR PUPIL

被引:34
|
作者
SHEPPARD, CJR
GU, M
机构
关键词
D O I
10.1016/0030-4018(91)90019-A
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Reported in this paper arc results on the improvement in axial resolution in scanning confocal microscopy when an annular pupil is combined with a finite-sized pinhole in front of the detector. Theoretical analyses show the optimum relationship of the axial resolution to the pinhole size and the radius of central obstruction. The magnitude of the signal level is also explored. The experimental results are well in agreement with the theoretical predictions.
引用
收藏
页码:7 / 13
页数:7
相关论文
共 50 条
  • [1] Phase pupil filters for improvement of the axial resolution in confocal scanning microscopy
    Kowalczyk, M
    Zapata, C
    Silvestre, E
    Martinez-Corral, M
    OPTICA APPLICATA, 1998, 28 (02) : 127 - 137
  • [2] IMPROVED AXIAL RESOLUTION IN CONFOCAL FLUORESCENCE MICROSCOPY USING ANNULAR PUPILS
    GU, M
    TANNOUS, T
    SHEPPARD, CJR
    OPTICS COMMUNICATIONS, 1994, 110 (5-6) : 533 - 539
  • [3] Improvement of axial resolution in confocal microscopy using heterodyne illumination
    Lee, SeungWoo
    Yoo, Hongki
    Chun, Byung S. Eon
    Chun, Wanhee
    Gweon, Dae-gab
    CONFOCAL, MULTIPHOTON, AND NONLINEAR MICROSCOPIC IMAGING III, 2007, 6630
  • [4] IMPROVEMENT IN AXIAL RESOLUTION BY INTERFERENCE CONFOCAL MICROSCOPY
    SHEPPARD, CJR
    GONG, YR
    OPTIK, 1991, 87 (03): : 129 - 132
  • [5] Improvement of axial resolution in confocal microscopy by an optical pupil filter with two zones phase-shifted by π
    Lin, L
    Wang, XH
    Wang, ZQ
    Mu, GG
    CHINESE PHYSICS LETTERS, 2003, 20 (09) : 1641 - 1643
  • [6] Differential confocal microscopy with annular pupil filter
    Liu, L
    Deng, XQ
    Yang, LS
    Wang, GY
    Xu, ZZ
    BIOMEDICAL PHOTONICS AND OPTOELECTRONIC IMAGING, 2000, 4224 : 73 - 77
  • [7] Improvement of the axial resolution in confocal microscopy by the use of heterodyne interference
    Lee, Seungwoo
    Gweon, Dae-Gab
    MEASUREMENT SCIENCE AND TECHNOLOGY, 2008, 19 (10)
  • [9] Effect of an annular pupil filter on differential confocal microscopy
    Liu, L
    Deng, XQ
    Yang, LS
    Wang, GY
    Xu, ZZ
    OPTICS LETTERS, 2000, 25 (23) : 1711 - 1713
  • [10] OPTIMIZATION OF AXIAL RESOLUTION IN CONFOCAL IMAGING USING ANNULAR PUPILS
    GU, M
    SHEPPARD, CJR
    ZHOU, H
    OPTIK, 1993, 93 (02): : 87 - 90