FURTHER CHARACTERIZATION OF THE INVITRO HYDROLYSIS OF [LEU]ENKEPHALIN AND [MET]ENKEPHALIN IN RAT PLASMA - HPLC-ECD MEASUREMENT OF SUBSTRATE AND METABOLITE CONCENTRATIONS

被引:23
|
作者
SHIBANOKI, S
WEINBERGER, SB
ISHIKAWA, K
MARTINEZ, JL
机构
[1] UNIV CALIF BERKELEY,DEPT PSYCHOL,3210 TOLMAN HALL,BERKELEY,CA 94720
[2] NIHON UNIV,SCH MED,DEPT PHARMACOL,TOKYO 101,JAPAN
关键词
HPLC-ECD; RAT PLASMA; ENKEPHALIN; HYDROLYSIS; AMINOPEPTIDASE-M; ANGIOTENSIN CONVERTING ENZYME;
D O I
10.1016/0167-0115(91)90020-H
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Hydrolysis of [Leu]- and [Met]enkephalin was determined in whole rat plasma in vitro by using HPLC-ECD to measure Tyr, Tyr-Gly and Tyr-Gly-Gly formation. Although [Leu]- and [Met]enkephalin did not differ in Tyr or Tyr-Gly accumulation, the amount of Tyr-Gly-Gly resulting from [Met]enkephalin hydrolysis was greater than that resulting from [Leu]enkephalin hydrolysis, and [Met]enkephalin's half-life in plasma was slightly shorter than that of [Leu]enkephalin. By comparing metabolite formation in the presence and absence of peptidase inhibitors with high selectivity for their respective enzymes, these studies demonstrated that aminopeptidase M and angiotensin converting enzyme are the major peptidases that hydrolyze enkephalins in rat plasma.
引用
收藏
页码:267 / 278
页数:12
相关论文
共 5 条
  • [1] HYDROLYSIS OF [LEU]ENKEPHALIN BY CHICK PLASMA INVITRO
    SHIBANOKI, S
    WEINBERGER, SB
    BENISTON, D
    NUDELMAN, KA
    SCHULTEIS, G
    BENNETT, EL
    ROSENZWEIG, MR
    ISHIKAWA, K
    MARTINEZ, JL
    [J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1991, 256 (02): : 650 - 655
  • [2] CHARACTERIZATION OF HYDROLYSIS OF [LEU]ENKEPHALIN AND D-ALA2-[L-LEU]ENKEPHALIN IN RAT PLASMA
    WEINBERGER, SB
    MARTINEZ, JL
    [J]. JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1988, 247 (01): : 129 - 135
  • [3] RELEASE OF THE HEPTAPEPTIDE MET-ENKEPHALIN-ARG6-PHE7 AND OF THE OCTAPEPTIDE MET-ENKEPHALIN-ARG6-GLY7-LEU8 FROM RAT STRIATUM INVITRO AND THEIR RAPID INACTIVATION
    PATEY, G
    CUPO, A
    CHAMINADE, M
    MORGAT, JL
    ROSSIER, J
    [J]. LIFE SCIENCES, 1983, 33 : 117 - 120
  • [4] DISTRIBUTION AND CHARACTERIZATION OF THE OPIOID OCTAPEPTIDE MET5-ENKEPHALIN-ARG6-GLY7-LEU8 IN THE GASTROINTESTINAL-TRACT OF THE RAT
    WANG, YN
    LINDBERG, I
    [J]. CELL AND TISSUE RESEARCH, 1986, 244 (01) : 77 - 85
  • [5] THE OPIOID OCTAPEPTIDE MET5-ENKEPHALIN-ARG6-GLY7-LEU8 - CHARACTERIZATION AND DISTRIBUTION IN RAT SPINAL-CORD
    IADAROLA, MJ
    PANULA, P
    MAJANE, EA
    YANG, HYT
    [J]. BRAIN RESEARCH, 1985, 330 (01) : 127 - 134