PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE-PEROXIDASE - FULL-LENGTH PIG BLASTOCYST CDNA SEQUENCE AND REGULATION BY SELENIUM STATUS

被引：61
|
作者：
SUNDE, RA [1 ]
DYER, JA [1 ]
MORAN, TV [1 ]
EVENSON, JK [1 ]
SUGIMOTO, M [1 ]
机构：
[1] UNIV MISSOURI,COLL AGR FOOD & NAT RESOURCES,DEPT BIOCHEM,COLUMBIA,MO 65211
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1993年 / 193卷 / 03期
关键词：
D O I：
10.1006/bbrc.1993.1711
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated and sequenced an apparent full-length cDNA clone for phospholipid hydroperoxide glutathione peroxidase (Genbank accession number L12743) from a pig blastocyst cDNA library. The sequence encodes a polypeptide of 170 amino acids, including a TGA-encoded selenocysteine at residue 46, with a calculated M(r) of 19,492 Da. Use of this clone in Northern blot analysis of Se-deficient rat liver revealed that phospholipid hydroperoxide glutathione peroxidase mRNA levels were little affected by Se deficiency, whereas classicial glutathione peroxidase mRNA levels were decreased by 90% in the same samples. Lastly, liver phospholipid hydroperoxide glutathione peroxidase mRNA levels were not elevated in female rats, in contrast to classical glutathione peroxidase. © 1993 Academic Press, Inc.
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页码：905 / 911
页数：7
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