DEVELOPMENT OF ELISA-BASED ASSAYS FOR DETECTION, QUANTITATION AND AVIDITY DETERMINATION OF SALIVARY IGA ANTIGLIADIN ANTIBODIES

Specific antibodies of IgA class can be detected in both sera and saliva of patients with hypersensitivity to wheat gliadin. This finding can be used for a non-invasive diagnostic procedure for celiac disease (CD). Development of an ELISA methods for detection, quantitation and measurement of avidity of salivary IgA anti-gladin is presented in this paper. A fast chromatographic procedure for isolation of standard secretory IgA is also presented. Comparing the avidity of rabbit IgG anti-BSA antibodies in serum samples taken during primary and secondary immune response with avidity of rabbit hyperimmune IgG antibodies as well as with human salivary IgA anti-gliadin, it was shown that gliadin induces synthesis of antibodies of low avidity only. Methods described in this paper will be used for further investigation of the role of mucosal anti-gliadin antibodies in the development of celiac disease.