STUDIES ON IMMOBILIZATION OF UREASE IN GELATIN BY CROSS-LINKING

被引：45
|
作者：
SUNGUR, S
ELCIN, YM
AKBULUT, U
机构：
[1] MIDDLE E TECH UNIV, DEPT CHEM, 06531 ANKARA, TURKEY
[2] ANKARA UNIV, DEPT CHEM, 06100 ANKARA, TURKEY
来源：
BIOMATERIALS | 1992年 / 13卷 / 11期
关键词：
IMMOBILIZATION; ENZYMES; UREASE; GELATIN;
D O I：
10.1016/0142-9612(92)90020-O
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Urease enzyme was immobilized in photographic gelatin by chemical cross-linking using formaldehyde, glutaraldehyde and chromium (III) acetate. The effects of enzyme and cross-linker concentrations, temperature, incubation time and pH on urea hydrolysis were investigated. Effect of reuse on the activity of immobilized enzyme was also studied. Glutaraldehyde (0.004 M) was the most suitable cross-linker; relative activities within 2.5 months after 24 reuses were stable (about 78%).
引用
收藏
页码：795 / 800
页数：6
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